Tall fescue seed extraction and partial purification of ergot alkaloids

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Tall fescue seed extraction and partial purification of ergot alkaloids

Many substances in the tall fescue/endophyte association (Schedonorus arundinaceus/Epichloë coenophiala) have biological activity. Of these compounds only the ergot alkaloids are known to have significant mammalian toxicity and the predominant ergot alkaloids are ergovaline and ergovalinine. Because synthetically produced ergovaline is difficult to obtain, we developed a seed extraction and par...

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Liver cytochrome P450 system as affected by endophyte-infected tall fescue seed extracts and ergot alkaloids

Endophyte infected tall fescue (E+) is the base diet for nearly all beef cattle in the southern USA. It has been linked to a variety of toxicological conditions due to the presence of large numbers of ergot alkaloids. This study was designed to investigate the effects of E+ seed extract and selected ergot alkaloids on the detoxification pathway by cytochrome P450 (CYP3A4) enzyme system. Tests w...

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Occurrence of peptide and clavine ergot alkaloids in tall fescue grass.

Evidence is presented that ergot alkaloids are ubiquitous in tall fescue pastures infected with the clavicipitaceous fungal endophyte Sphacelia typhina (or Acremonium coenophialum). Ergopeptide alkaloids, predominantly ergovaline, constituted 10 to 50 percent of the total ergot alkaloid concentration, which was as high as 14 milligrams per kilogram in sheaths and 1.5 milligrams per kilogram in ...

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Procedure for isolating the endophyte from tall fescue and screening isolates for ergot alkaloids.

A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolated mycelium and characteristic conidia within a 3- to 3.5-week period. Once is...

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Ergopeptine Alkaloids in Grazed Tall Fescue

Endophyte (Acremonium cotnophiaJum Morgan-Jones et Gams) -Infected tall fescue (Festuca arundinacea Schreb.) produces ergopeptiDe alkaloids (EP) that may be associated with fescue toxicosis symptoms in cattle (B03 taurus). A study was conducted during 1983 and 1984 at Watkinsville, GA, to determine the effects of endophyte infection, season, and N fertilization on EP concentration and Steer ave...

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ژورنال

عنوان ژورنال: Frontiers in Chemistry

سال: 2014

ISSN: 2296-2646

DOI: 10.3389/fchem.2014.00110